DSBs can be repaired using several different mechanisms. Both ends can be simply rejoined with little or no further processing (nonhomologous end joining, or NHEJ) or can be repaired using homologous ...
Most biochemistry labs that study DNA isolate it within a water-based solution that allows scientists to manipulate DNA without interacting with other molecules. They also tend to use heat to separate ...
When a double strand break, or DSB -- the most severe form of DNA damage -- occurs, the repair pathway may insert or delete genes at the break site or potentially rearrange genes throughout the strand ...
One of the hallmarks of DNA damage is the rapid spreading of phosphorylated histone H2A (γ-H2AX) around a DNA double-strand break (DSB). In the budding yeast Saccharomyces cerevisiae, nearly all H2A ...
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