Genome editing using designer nucleases such as transcription activator-like effector nucleases (TALENs) or clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 nucleases is an ...

Single-cell quantitative real-time PCR (qRT-PCR) combined with high-throughput arrays allows the analysis of gene expression profiles at a molecular level in approximately 11 h after cell sample ...

When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

By Dr. Liji Thomas, MD A new same-day detection strategy shows how simple, low-cost lab steps can uncover Salmonella in meats ...

Setting: The diagnosis of extra-pulmonary tuberculosis (EPTB) remains an important clinical problem, primarily because of the inadequate sensitivity of conventional bacteriologic methods for detecting ...

Gene-Analysis Studies Advanced by Use of Low-Volume High-Throughput Real-Time PCR This article describes three gene-analysis studies using the Roche LightCycler® 1536 Real-Time PCR System (Figure 1).

A reverse-transcriptase-polymerase-chain-reaction (RT-PCR) procedure was evaluated for detection of chicken proventricular necrosis virus (CPNV) in transmissible viral proventriculitis (TYP) -affected ...

This is a preview. Log in through your library . Abstract A rapid diagnostic method employing a polymerase chain reaction procedure (PCR) was used to identify Shigella and enteroinvasive Escherichia ...

In the six decades since Watson and Crick identified the double helical structure of DNA, a series of elegant and powerful experiments has established the broad outlines of how genetic information is ...

The heel-stick procedure, a routine screening test for several metabolic and genetic disorders in newborns, is not effective in screening for cytomegalovirus (CMV) infection, a leading cause of ...

Researchers at the German Red Cross Blood Donor Service in Frankfurt headed by Professor Erhard Seifried, and the Institute for Medical Virology at the University Hospital Frankfurt at Goethe ...