Here we describe the generation of a double-fluorescent Cre/loxP indicator system. This protocol involves (i) all cloning steps to generate the plasmid vector (3–5 months); (ii) a guide to prepare ...
CHICAGO--(BUSINESS WIRE)--GSL Biotech and VectorBuilder have formed a partnership to create a complete cloning solution, allowing customers to order their entire custom plasmids directly from ...
Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...
Processes yielding >500 mg plasmid DNA/L have previously been reported by Merck, Boehringer Ingelheim, and Nature Technology (NTC). Common features among these high-yield processes include the use of ...
Bacterial cells often possess molecules of closed, circular DNA, otherwise known as ‘plasmids’. They can also be present at much lower frequencies in certain eukaryotic cell types, such as yeast. They ...
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