The highly purified restriction endonucleases of E. coli K and coliphage P1 transfer methyl groups from S-adenosylmethionine to adenine residues of unmodified DNA. Incubation of unmodified DNA with ...

Modular cloning and expression of complex genetic assemblies are becoming widely popular. Recent applications based on site-specific and homologous recombination have substantially improved the ...

Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...

The basic technology of genetic engineering is artificial cutting, splicing, and combination of genes. A gene is a DNA molecule with a certain function. To accurately cut out the DNA linear molecular ...

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...

DNA methylation is one of the most important modifications in mammalian cells which plays an essential role in regulating growth of the cells and their proliferation. In humans, it refers to the ...

To many working biologists, DNA-modifying enzymes are tools, not unlike a pair of molecule-sized scissors. To Dr. Barry Stoddard, a professor in the Basic Sciences division at Fred Hutch, these ...