Scientists first observed the biological phenomenon of restriction and modification in the 1950s, but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton ...
Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...
Molecular cloning relies on two key components to construct recombinant DNA: 1) a DNA vector and 2) one or more DNA fragments containing the gene(s) of interest (GOI). To produce a GOI fragment, DNA ...
Developments in molecular diagnostics have been vital in discovering novel pathogens and diagnosing many human diseases. The recent coronavirus 2019 (COVID-19) pandemic, caused by severe acute ...
Lead author Kathleen A. Christie, PhD, postdoctoral researcher at Harvard Medical School; research fellow at Massachusetts General Hospital. If you’ve ever spent time in a molecular biology lab, there ...
A popular experiment that's recommended to demonstrate the functionality of enzymes is chewing a piece of bread for a moment, then paying attention to when it turns from a starchy flavor to begin ...
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