Here, we describe a method of systematic PCR screening with multiround sample pooling for the isolation of rare PCR-positive samples. As an example, we have applied this protocol to the recovery of ...

When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

Association of Notch-1 phenotype with clinical stage progression in non-small cell lung cancer. This is an ASCO Meeting Abstract from the 2014 ASCO Annual Meeting I. This abstract does not include a ...

The polymerase chain reaction (PCR) technique continues to grow and develop, now boasting several different types, having a variety of effective applications such as the detection of pathogens and ...

In a recently published study in BioTechniques, researchers have reassessed the status quo by performing a direct comparison on single versus triplicate 16S PCR reactions across hundreds of samples in ...

Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. Post editing, it is critical to provide precise and accurate quantification for ...

In a recent study posted to the medRxiv* preprint server, researchers examined sample collection practices, epidemiological characteristics, and cycle threshold (Ct) values for polymerase chain ...

With more cases of patients showing clinical symptoms of Covid-19 but RT-PCR tests coming back negative, hospitals have laid down new protocols to stop emerging variants from going undetected. In a ...

Mullis used the basis of Sanger's DNA sequencing as a starting point for his new technique. He realized that the repeated use of DNA polymerase triggered a chain reaction resulting in a specific DNA ...

IIT Bombay researchers have developed an algorithm which can be used to help conduct pooled testing of samples for COVID-19. In order to validate this protocol – known as Tapestry Pooling – in vitro ...

Health workers take PCR swab samples from students at SDN 025 Cikutra, Bandung, West Java, October 22, 2021. From a population of 1,200 children, 50 students at SDN Cikutra grades 5 and 6 were taken ...