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1:31
YouTube
BMH learning
Real Time PCR Using Taqman Probe | RT PCR using Taqman Dye |
An oligonucleotide probe called taqman probe is constructed containing a fluorescent reporter dye on the 5' end and a quencher dye on the 3' end. While the probe is intact, the proximity of the quencher dye greatly reduces the fluorescence emitted by the reporter dye by FRET. If the target sequence is present, the probe anneals downstream from ...
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